Sacked Catalan leader Carles Puigdemont has called for “democratic opposition” to direct rule from Madrid.
He condemned the suspension of Catalonia’s autonomy and promised to continue to “work to build a free country”.
He made the call in a pre-recorded TV address to Catalans broadcast on Saturday afternoon.
The Spanish government has stripped Catalonia of its autonomy and taken charge of its government.
The measures came early on Saturday after the Catalan parliament voted to declare independence the previous day
Earlier, Spain’s interior ministry took charge of Catalonia’s police after firing senior Catalan police officials.
Friday saw Spanish Prime Minister Mariano Rajoy announce the dissolution of the regional parliament and the removal of Mr Puigdemont as Catalan leader, and call snap regional elections.
An an official state bulletin (in Spanish) handed control of Catalonia to Spain’s Deputy Prime Minister, Soraya Saenz de Santamaria.
Demonstrations for and against independence went on into the night and a large rally “for the unity of Spain and the constitution” was held in Madrid on Saturday.
In his TV address, Mr Puigdemont, describing the declaration of independence, said Friday had been “a day with democratic and civic sensibility”.
He said the central government’s actions since then were “contrary to the expressed will of the citizens of our country, who know perfectly well that in a democracy it is parliaments that choose, or remove, presidents”.
The Catalan government said that of the 43% of potential voters who took part, 90% were in favour of independence.
Before Madrid took over the Catalan government, the region had one of the greatest levels of self-government in Spain.
It has its own parliament, police force and public broadcaster, as well as a government and president, though those have now been dismissed.
Catalans had a range of powers in many policy areas from culture and environment to communications, transportation, commerce and public safety.
Foreign affairs, the armed forces and fiscal policy were always the sole responsibility of the Spanish government.
Thousands celebrated the declaration of independence on the streets of Barcelona, Catalonia’s regional capital.
The same crowds that cheered each Yes vote from Catalan MPs reportedly booed Mr Rajoy as he made his announcement.
There were pro-unity demonstrations too, with protesters in Barcelona waving Spanish flags and denouncing Catalan independence. A large pro-unity rally is expected in Madrid on Saturday.
In other reaction:
After the 1 October referendum, Mr Puigdemont signed a declaration of independence but delayed implementation to allow talks with the Spanish government.
He ignored warnings by the Madrid government to cancel the move, prompting Mr Rajoy to first announce his plans to remove Catalan leaders and impose direct rule.
Catalonia is one of Spain’s richest, most distinctive regions, with a high degree of autonomy.
Many Catalans feel they pay more to Madrid than they get back, and there are historical grievances, too, in particular Catalonia’s treatment under the dictatorship of General Franco. But Catalans have been divided on the question of independence.